![]() ![]() Densitometric analysis of the Western blots indicated that the linear range for IB was 0.0973. The Western blot assay is a method in which individual proteins of an HIV-1 lysate are separated according to size by polyacrylamide gel electrophoresis. The ELISAs were performed according to the manufacturers instructions. The purpose of this report is to provide guidance for interpreting Western blot test results and their use in diagnosing HIV-1 infection. Frequency, causes, and new challenges of indeterminate results in Western blot confirmatory testing for antibodies to human immunodeficiency virus. The quantitative Western blot data were confirmed using p53 and IB ELISA assays (Assay Designs, Ann Arbor, MI). 2018 2018:4390318. doi:10.1155/2018/4390318Ĭenters for Disease Control and Prevention. It is an Immunoblotting technique that relies on the specificity of binding between a molecule of interest and a probe to. Western blot-based logistic regression model for the identification of recent HIV-1 infection: A promising HIV-1 surveillance approach for resource-limited regions. ![]() Laboratory Testing for the Diagnosis of HIV Infection: Updated Recommendations. doi:10.1016/j.jcv.2013.10.006Ĭenters for Disease Control and Prevention and Association of Public Health Laboratories. The Multispot rapid HIV-1/HIV-2 differentiation assay is comparable with the Western blot and an immunofluorescence assay at confirming HIV infection in a prospective study in three regions of the United States. ![]()
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